产品描述
This Rabbit KL-6 ELISA Kit was designed for the quantitative measurement of Rabbit KL-6 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 50 IU/mL-800 IU/mL and the sensitivity is 25 IU/mL .
缩写
KL-6
灵敏度
25 IU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<15% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit KL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-93 | |
| 1:2 | Average % | 94 | |
| Range % | 89-99 | |
| 1:4 | Average % | 92 | |
| Range % | 87-96 | |
| 1:8 | Average % | 95 | |
| Range % | 90-100 | |
回收率
| The recovery of rabbit KL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-99 | |
| EDTA plasma (n=4) | 89 | 87-93 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| IU/ml | OD1 | OD2 | Average | | | | 800 | 0.191 | 0.198 | 0.195 | | | | 400 | 0.351 | 0.337 | 0.344 | | | | 200 | 0.614 | 0.627 | 0.621 | | | | 100 | 1.154 | 1.132 | 1.143 | | | | 50 | 2.330 | 2.224 | 2.277 | | |
|
货期
3-5 working days