产品描述

This human IL-32 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human IL-32 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the IL-32 levels in the sample and is measured at 450 nm through a microplate reader.

IL-32 is a pro-inflammatory cytokine that is identified as an immunoregulator that controls cell differentiation and cell death and is involved in the stimulation of anti−/pro-inflammatory cytokines. It is inducibly expressed in response to cellular stress such as hypoxia, infections, and pro-inflammatory cytokines. It plays a crucial role in host defense against pathogens, as well as in the pathogenesis of chronic inflammation. Aberrant IL-32 expression has been associated with several autoimmune diseases such as rheumatoid arthritis, inflammatory bowel diseases, and type 1 diabetes, as well as allergic and infectious diseases, and cancer.

别名

IL 32alpha ELISA Kit; IL 32beta ELISA Kit; IL 32delta ELISA Kit; IL 32gamma ELISA Kit; IL-32 ELISA Kit; IL32 ELISA Kit; IL32_HUMAN ELISA Kit; Interleukin 32 ELISA Kit; Interleukin 32 small ELISA Kit; Interleukin 32 theta ELISA Kit; Interleukin-32 ELISA Kit; interleukin-32 eta ELISA Kit; Natural killer cell transcript 4 ELISA Kit; Natural killer cells protein 4 ELISA Kit; NK 4 ELISA Kit; NK4 ELISA Kit; OTTHUMP00000236040 ELISA Kit; OTTHUMP00000236041 ELISA Kit; OTTHUMP00000236042 ELISA Kit; OTTHUMP00000236043 ELISA Kit; OTTHUMP00000236044 ELISA Kit; OTTHUMP00000236045 ELISA Kit; OTTHUMP00000236046 ELISA Kit; OTTHUMP00000236047 ELISA Kit; OTTHUMP00000236048 ELISA Kit; OTTHUMP00000236049 ELISA Kit; OTTHUMP00000236050 ELISA Kit; OTTHUMP00000236051 ELISA Kit; OTTHUMP00000236052 ELISA Kit; OTTHUMP00000236053 ELISA Kit; OTTHUMP00000236054 ELISA Kit; OTTHUMP00000241545 ELISA Kit; OTTHUMP00000241602 ELISA Kit; OTTHUMP00000241603 ELISA Kit; OTTHUMP00000241604 ELISA Kit; OTTHUMP00000241605 ELISA Kit; OTTHUMP00000241606 ELISA Kit; OTTHUMP00000241607 ELISA Kit; OTTHUMP00000241608 ELISA Kit; OTTHUMP00000241609 ELISA Kit; OTTHUMP00000241610 ELISA Kit; OTTHUMP00000241611 ELISA Kit; OTTHUMP00000241612 ELISA Kit; TAIF ELISA Kit; TAIFa ELISA Kit; TAIFb ELISA Kit; TAIFc ELISA Kit; TAIFd ELISA Kit; Tumor necrosis factor alpha-inducing factor ELISA Kit

缩写

IL32

Uniprot No

P24001

灵敏度

78.125 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IL-32 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
Range %	82-97
1:2	Average %	98
Range %	94-104
1:4	Average %	97
Range %	90-105
1:8	Average %	87
Range %	82-92

回收率

The recovery of human GROα spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		87-97
EDTA plasma (n=4)		101		96-106

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected   20000 2.801 2.752 2.777 2.627   10000 2.440 2.279 2.360 2.210   5000 1.784 1.789 1.787 1.637   2500 0.961 1.002 0.982 0.832   1250 0.547 0.575 0.561 0.411   625 0.338 0.346 0.342 0.192   312.5 0.234 0.225 0.230 0.080   0 0.146 0.154 0.150

货期

3-5 working days