产品描述
This mouse eNOS ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of mouse eNOS in multiple samples, including serum, plasma, and tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the eNOS levels in the sample and is measured at 450 nm through a microplate reader.
eNOS, also called NOS3, is usually constitutively expressed in cells in an inactive state and is activated by binding to the CaM protein after the elevation of Ca2+ concentration. NOS3 is highly enriched in endothelial cells (ECs) and contributes to the generation of nitric oxide (NO) to exert vasodilation and regulate the flow of blood throughout the body. It is linked to cardiovascular diseases such as hypertension, atherosclerosis, and diabetes mellitus. Recent studies have shown that NOS3 plays an important role in cancers, such as inhibiting apoptosis and promoting angiogenesis, proliferation, invasiveness, and immunosuppression.
别名
Nos3 ELISA Kit; EcnosNitric oxide synthase ELISA Kit; endothelial ELISA Kit; EC 1.14.13.39 ELISA Kit; Constitutive NOS ELISA Kit; cNOS ELISA Kit; EC-NOS ELISA Kit; Endothelial NOS ELISA Kit; eNOS ELISA Kit; NOS type III ELISA Kit; NOSIII ELISA Kit
缩写
NOS3
Uniprot No
P70313
灵敏度
0.078 IU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse eNOS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
86 |
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| Range % |
82-95 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
105 |
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| Range % |
98-112 |
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| 1:8 |
Average % |
98 |
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| Range % |
94-102 |
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回收率
| The recovery of mouse eNOS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
92-104 |
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| EDTA plasma (n=4) |
102 |
94-106 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| IU/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.995 |
2.865 |
2.930 |
2.728 |
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| 10 |
2.280 |
2.244 |
2.262 |
2.060 |
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| 5 |
1.528 |
1.546 |
1.537 |
1.335 |
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| 2.5 |
0.926 |
0.940 |
0.933 |
0.731 |
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| 1.25 |
0.598 |
0.578 |
0.588 |
0.386 |
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| 0.625 |
0.445 |
0.431 |
0.438 |
0.236 |
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| 0.312 |
0.298 |
0.305 |
0.302 |
0.100 |
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| 0 |
0.199 |
0.204 |
0.202 |
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货期
3-5 working days
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