产品描述

This human ITGAM ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human ITGAM in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the ITGAM levels in the sample and is measured at 450 nm through a microplate reader.

ITGAM is the encoding gene for CD11b, highly expressed on the surface of innate immune cells, including macrophages and neutrophils, which together with CD18, form Mac-1 or CR3, a protein that mediates leukocyte adhesion, migration, and phagocytosis in different cells including neutrophils, monocytes, macrophages, and dendritic cells. CD11b also contributes to the phagocytosis of opsonized particles, including apoptotic cells and the immune complex. It has consistently been associated with susceptibility to systemic lupus erythematosus (SLE).

别名

antigen CD11b (p170) ELISA Kit; Antigen CD11b p170 ELISA Kit; CD11 antigen like family member B ELISA Kit; CD11 antigen-like family member B ELISA Kit; CD11b ELISA Kit; CD11b/CD18 ELISA Kit; CD49d ELISA Kit; Cell surface glycoprotein MAC-1 subunit alpha ELISA Kit; Complement component 3 receptor 3 subunit ELISA Kit; Complement Component Receptor 3 Alpha ELISA Kit; Complement receptor type 3 ELISA Kit; Complement receptor type 3, alpha subunit ELISA Kit; CR 3 alpha chain (CR3A) ELISA Kit; CR 3 alpha chain ELISA Kit; CR-3 alpha chain ELISA Kit; CR3 ELISA Kit; CR3A ELISA Kit; F730045J24Rik ELISA Kit; Integrin Alpha M ELISA Kit; Integrin alpha M chain ELISA Kit; Integrin alpha-M ELISA Kit; Integrin beta 2 alpha subunit ELISA Kit; Integrin subunit alpha M ELISA Kit; integrin, alpha M (complement component 3 receptor 3 subunit) ELISA Kit; ITAM_HUMAN ELISA Kit; ITGAM ELISA Kit; Leukocyte adhesion receptor MO1 ELISA Kit; Ly-40 ELISA Kit; MAC 1 ELISA Kit; Mac-1a ELISA Kit; MAC1 ELISA Kit; Mac1, alpha subunit ELISA Kit; MAC1A ELISA Kit; Macrophage antigen alpha polypeptide ELISA Kit; MGC117044 ELISA Kit; Mo1 ELISA Kit; Mo1, alpha subunit ELISA Kit; MO1A ELISA Kit; Neutrophil adherence receptor alpha M subunit ELISA Kit; Neutrophil adherence receptor ELISA Kit; SLEB6 ELISA Kit

缩写

ITGAM

Uniprot No

P11215

灵敏度

0.195 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ITG/CD11b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		100
		Range %		95-104
1:2		Average %		98
		Range %		94-102
1:4		Average %		92
		Range %		87-98
1:8		Average %		89
		Range %		85-96

回收率

The recovery of human ITG/CD11b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		92		89-94
EDTA plasma (n=4)		96		92-102

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 50 2.439 2.569 2.504 2.320 25 1.933 1.987 1.960 1.776 12.5 1.485 1.498 1.492 1.308 6.25 0.999 1.009 1.004 0.820 3.12 0.575 0.597 0.586 0.402 1.56 0.394 0.374 0.384 0.200 0.78 0.308 0.316 0.312 0.128 0 0.183 0.185 0.184

货期

3-5 working days