产品描述
MAU/ALB(Microalbuminuria/Albumin)是检测肾脏损伤和糖尿病肾病的常用指标。MAU是尿液中微量白蛋白的浓度,高水平提示肾小球滤过膜损伤。ALB是尿液中白蛋白的浓度,高水平提示肾小管受损。对于糖尿病患者来说,MAU/ALB水平的监测可以帮助早期发现并治疗糖尿病肾病。
华美生物所提供的Rat microalbunminuria(MAU/ALB) ELISA kit属于ELISA检测试剂盒,采用双抗夹心法定量检测大鼠血清、血浆、尿液样本中的MAU/ALB,其灵敏度为0.01 μg/mL,检测范围为0.01 μg/mL-40 μg/mL。
别名
Alb 1 ELISA Kit; alb ELISA Kit; ALBU_RAT ELISA Kit; Albumin ELISA Kit; Serum albumin ELISA Kit
缩写
MAU/ALB
Uniprot No
P02770
灵敏度
0.01 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MAU in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
93 |
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| Range % |
85-107 |
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| 1:2000 |
Average % |
95 |
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| Range % |
87-104 |
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| 1:4000 |
Average % |
89 |
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| Range % |
84-100 |
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| 1:8000 |
Average % |
90 |
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| Range % |
81-102 |
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回收率
| The recovery of rat MAU spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
88-97 |
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| EDTA plasma (n=4) |
97 |
90-105 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.345 |
2.412 |
2.379 |
2.279 |
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| 10 |
2.176 |
2.210 |
2.193 |
2.093 |
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| 2.5 |
1.682 |
1.657 |
1.670 |
1.570 |
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| 0.625 |
1.237 |
1.253 |
1.245 |
1.145 |
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| 0.156 |
0.783 |
0.802 |
0.793 |
0.693 |
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| 0.039 |
0.429 |
0.436 |
0.433 |
0.333 |
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| 0.01 |
0.272 |
0.281 |
0.277 |
0.177 |
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| 0 |
0.101 |
0.098 |
0.100 |
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货期
3-5 working days