产品描述
This Human MPZ ELISA Kit was designed for the quantitative measurement of Human MPZ protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
Charcot Marie Tooth neuropathy 1B ELISA Kit; CHM ELISA Kit; CMT1 ELISA Kit; CMT1B ELISA Kit; CMT2I ELISA Kit; CMT2J ELISA Kit; CMT4E ELISA Kit; CMTDI3 ELISA Kit; CMTDID ELISA Kit; DSS ELISA Kit; HMSNIB ELISA Kit; MPP ELISA Kit; MPZ ELISA Kit; Myelin peripheral protein ELISA Kit; Myelin protein P0 ELISA Kit; Myelin protein zero ELISA Kit; MYP0_HUMAN ELISA Kit; P0 ELISA Kit
缩写
MPZ
Uniprot No
P25189
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MPZ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 92 | | |
| Range % | 88-96 | | |
| 1:2 | Average % | 96 | | |
| Range % | 92-100 | | |
| 1:4 | Average % | 89 | | |
| Range % | 85-93 | | |
| 1:8 | Average % | 94 | | |
| Range % | 90-97 | | |
回收率
| The recovery of human MPZ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 106 | 101-112 | | |
| EDTA plasma (n=4) | 97 | 92-101 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | | 2000 | 2.493 | 2.516 | 2.505 | 2.376 | | | | 1000 | 2.284 | 2.101 | 2.193 | 2.064 | | | | 500 | 1.715 | 1.753 | 1.734 | 1.605 | | | | 250 | 1.271 | 1.243 | 1.257 | 1.128 | | | | 125 | 0.849 | 0.812 | 0.831 | 0.702 | | | | 62.5 | 0.542 | 0.579 | 0.561 | 0.432 | | | | 31.25 | 0.327 | 0.344 | 0.336 | 0.207 | | | | 0 | 0.130 | 0.127 | 0.129 | | | |
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货期
3-5 working days