产品描述
This Pig MUC2 ELISA Kit was designed for the quantitative measurement of Pig MUC2 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 ng/mL-500 ng/mL and the sensitivity is 1.95 ng/mL.
缩写
MUC2
灵敏度
1.95 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig mucin-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 91 | |
| Range % | 87-94 | |
| 1:2 | Average % | 97 | |
| Range % | 92-101 | |
| 1:4 | Average % | 90 | |
| Range % | 87-98 | |
| 1:8 | Average % | 92 | |
| Range % | 85-95 | |
回收率
| The recovery of pig mucin-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 93 | 88-96 | |
| EDTA plasma (n=4) | 98 | 95-102 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 500 | 1.976 | 2.121 | 2.049 | 1.885 | | | 250 | 1.303 | 1.210 | 1.257 | 1.093 | | | 125 | 0.774 | 0.784 | 0.779 | 0.615 | | | 62.5 | 0.428 | 0.425 | 0.427 | 0.263 | | | 31.25 | 0.296 | 0.274 | 0.285 | 0.121 | | | 15.62 | 0.235 | 0.250 | 0.243 | 0.079 | | | 7.8 | 0.208 | 0.207 | 0.208 | 0.044 | | | 0 | 0.158 | 0.169 | 0.164 | | |
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货期
3-5 working days