产品描述
This Human MUC2 ELISA Kit was designed for the quantitative measurement of Human MUC2 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 ng/mL-200 ng/mL and the sensitivity is 0.78 ng/mL .
别名
Intestinal mucin 2 ELISA Kit; Intestinal mucin-2 ELISA Kit; MLP ELISA Kit; MUC 2 ELISA Kit; MUC-2 ELISA Kit; Muc2 ELISA Kit; MUC2_HUMAN ELISA Kit; Mucin 2 ELISA Kit; Mucin 2 intestinal ELISA Kit; Mucin 2 intestinal/tracheal ELISA Kit; Mucin 2 oligomeric mucus/gel forming ELISA Kit; Mucin 2 precursor ELISA Kit; Mucin 2 tracheal ELISA Kit; Mucin like protein ELISA Kit; Mucin-2 ELISA Kit; Mucin2 ELISA Kit; SMUC ELISA Kit
缩写
MUC2
Uniprot No
Q02817
灵敏度
0.78 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human MUC2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 87-96 | |
| 1:2 | Average % | 101 | |
| Range % | 99-105 | |
| 1:4 | Average % | 94 | |
| Range % | 90-98 | |
| 1:8 | Average % | 89 | |
| Range % | 85-94 | |
回收率
| The recovery of human MUC2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 84 | 80-88 | |
| EDTA plasma (n=4) | 95 | 90-99 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 200 | 2.531 | 2.481 | 2.506 | 2.364 | | | 100 | 2.032 | 1.961 | 1.997 | 1.855 | | | 50 | 1.448 | 1.401 | 1.425 | 1.283 | | | 25 | 0.876 | 0.823 | 0.850 | 0.708 | | | 12.5 | 0.597 | 0.623 | 0.610 | 0.468 | | | 6.25 | 0.391 | 0.402 | 0.397 | 0.255 | | | 3.12 | 0.233 | 0.217 | 0.225 | 0.083 | | | 0 | 0.144 | 0.139 | 0.142 | | |
|
货期
3-5 working days