产品描述
This Rat NTF4 ELISA Kit was designed for the quantitative measurement of Rat NTF4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 47 pg/mL-3000 pg/mL and the sensitivity is 11.75 pg/mL .
别名
Ntf4 ELISA Kit; Nt4 ELISA Kit; Ntf5 ELISA Kit; Neurotrophin-4 ELISA Kit; NT-4 ELISA Kit; Neurotrophin-5 ELISA Kit; NT-5 ELISA Kit; Neutrophic factor 4 ELISA Kit
缩写
NTF4
Uniprot No
P34131
灵敏度
11.75 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 86 | |
| Range % | 82-94 | |
| 1:2 | Average % | 93 | |
| Range % | 88-98 | |
| 1:4 | Average % | 96 | |
| Range % | 90-102 | |
| 1:8 | Average % | 99 | |
| Range % | 95-104 | |
回收率
| The recovery of rat NT-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-102 | |
| EDTA plasma (n=4) | 100 | 95-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 3000 | 2.268 | 2.250 | 2.259 | 2.150 | | | 1500 | 1.845 | 1.871 | 1.858 | 1.749 | | | 750 | 1.464 | 1.492 | 1.478 | 1.369 | | | 375 | 1.135 | 1.149 | 1.142 | 1.033 | | | 187.5 | 0.704 | 0.724 | 0.714 | 0.605 | | | 94 | 0.438 | 0.457 | 0.448 | 0.339 | | | 47 | 0.287 | 0.294 | 0.291 | 0.182 | | | 0 | 0.108 | 0.110 | 0.109 | | |
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货期
3-5 working days