产品描述
IL18(白细胞介素18)是一种由免疫细胞产生的细胞因子,在免疫系统中具有重要作用。IL18可促进T细胞的增殖和活化,同时也能促进NK细胞和单核细胞的活化和产生促炎症因子。IL18也参与机体对细菌、病毒和真菌等病原体的免疫应答。IL18在健康人体内的正常含量为0-130pg/mL,但其含量会因各种因素如炎症等而发生变化。
华美生物所提供的Human Interleukin 18,IL-18 ELISA KIT属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、尿液、组织匀浆样本中的IL18,其灵敏度为7.8 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
Iboctadekin ELISA Kit; IFN gamma inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; IGIF ELISA Kit; IL 1 gamma ELISA Kit; IL 18 ELISA Kit; IL 1g ELISA Kit; IL-1 gamma ELISA Kit; IL-18 ELISA Kit; IL1 gamma ELISA Kit; IL18 ELISA Kit; IL18 protein ELISA Kit; IL18_HUMAN ELISA Kit; IL1F4 ELISA Kit; IL1g ELISA Kit; IL1gamma ELISA Kit; ILIF4 ELISA Kit; Interferon gamma inducing factor ELISA Kit; Interferon gamma-inducing factor ELISA Kit; Interleukin 1 gamma ELISA Kit; Interleukin 18 (interferon-gamma-inducing factor) ELISA Kit; Interleukin 18 ELISA Kit; Interleukin-1 gamma ELISA Kit; Interleukin-18 ELISA Kit; Interleukin18 ELISA Kit; MGC12320 ELISA Kit
缩写
IL18
Uniprot No
Q14116
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
91-99 |
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| 1:2 |
Average % |
105 |
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| Range % |
99-108 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-98 |
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| 1:8 |
Average % |
96 |
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| Range % |
91-102 |
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回收率
| The recovery of human IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
99 |
94-102 |
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| EDTA plasma (n=4) |
92 |
87-95 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
1.956 |
1.897 |
1.927 |
1.844 |
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| 1000 |
1.412 |
1.424 |
1.418 |
1.335 |
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| 500 |
0.876 |
0.853 |
0.865 |
0.782 |
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| 250 |
0.442 |
0.465 |
0.454 |
0.371 |
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| 125 |
0.310 |
0.294 |
0.302 |
0.219 |
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| 62.5 |
0.171 |
0.179 |
0.175 |
0.092 |
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| 31.25 |
0.119 |
0.123 |
0.121 |
0.038 |
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| 0 |
0.081 |
0.085 |
0.083 |
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货期
3-5 working days