产品描述

This Human IRAK4 ELISA Kit was designed for the quantitative measurement of Human IRAK4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 6.25 pg/mL-400 pg/mL and the sensitivity is 1.56 pg/mL.

别名

IL-1 receptor-associated kinase 4 ELISA Kit; Interleukin 1 receptor associated kinase 4 mutant form 1 ELISA Kit; Interleukin-1 receptor-associated kinase 4 ELISA Kit; Interleukin1 receptor associated kinase 4 ELISA Kit; IPD1 ELISA Kit; IRAK 4 ELISA Kit; IRAK-4 ELISA Kit; IRAK4 ELISA Kit; IRAK4 mutated form 1 ELISA Kit; IRAK4_HUMAN ELISA Kit; LOC 51135 ELISA Kit; NY REN 64 ELISA Kit; NY REN 64 antigen ELISA Kit; NY-REN-64 ELISA Kit; REN64 ELISA Kit; Renal carcinoma antigen NY-REN-64 ELISA Kit

缩写

IRAK4

Uniprot No

Q9NWZ3

灵敏度

1.56 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IRAK-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		92
		Range %		86-96
1:10		Average %		107
		Range %		103-111
1:20		Average %		92
		Range %		87-98
1:40		Average %		88
		Range %		84-93

回收率

The recovery of human IRAK-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		87		83-92
EDTA plasma (n=4)		102		97-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 400 2.987 2.920 2.954 2.867 200 2.485 2.315 2.400 2.313 100 1.578 1.564 1.571 1.484 50 0.852 0.843 0.848 0.761 25 0.473 0.467 0.470 0.383 12.5 0.255 0.245 0.250 0.163 6.25 0.169 0.161 0.165 0.078 0 0.087 0.086 0.087

货期

3-5 working days