产品描述
This Rat Orexin A ELISA Kit was designed for the quantitative measurement of Rat Orexin A protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
别名
Hcrt ELISA Kit; Ox ELISA Kit; Ppox ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit
缩写
Orexin A
Uniprot No
O55232
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 84-107 | |
| 1:2 | Average % | 92 | |
| Range % | 86-99 | |
| 1:4 | Average % | 95 | |
| Range % | 91-99 | |
| 1:8 | Average % | 96 | |
| Range % | 90-103 | |
回收率
| The recovery of rat orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 89-101 | |
| EDTA plasma (n=4) | 88 | 83-95 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.108 | 2.076 | 2.092 | 1.978 | | | 500 | 1.774 | 1.785 | 1.780 | 1.666 | | | 250 | 1.442 | 1.468 | 1.455 | 1.341 | | | 125 | 1.140 | 1.149 | 1.145 | 1.031 | | | 62.5 | 0.744 | 0.763 | 0.754 | 0.640 | | | 31.2 | 0.525 | 0.534 | 0.530 | 0.416 | | | 15.6 | 0.291 | 0.282 | 0.287 | 0.173 | | | 0 | 0.113 | 0.114 | 0.114 | | |
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货期
3-5 working days