产品描述

This Human KEAP1 ELISA Kit was designed for the quantitative measurement of Human KEAP1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 25 pg/mL-1600 pg/mL and the sensitivity is 6.25 pg/mL.

别名

Cytosolic inhibitor of Nrf2 ELISA Kit; INrf 2 ELISA Kit; INrf2 ELISA Kit; Keap 1 ELISA Kit; KEAP1 ELISA Kit; KEAP1_HUMAN ELISA Kit; Kelch like ECH associated protein 1 ELISA Kit; Kelch like family member 19 ELISA Kit; Kelch like protein 19 ELISA Kit; Kelch-like ECH-associated protein 1 ELISA Kit; Kelch-like protein 19 ELISA Kit; KIAA0132 ELISA Kit; KLHL 19 ELISA Kit; KLHL19 ELISA Kit; MGC10630 ELISA Kit; MGC1114 ELISA Kit; MGC20887 ELISA Kit; MGC4407 ELISA Kit; MGC9454 ELISA Kit

缩写

KEAP1

Uniprot No

Q14145

灵敏度

6.25 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Epigenetics and Nuclear Signaling

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KEAP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		89-96
1:2		Average %		97
		Range %		93-103
1:4		Average %		95
		Range %		89-99
1:8		Average %		92
		Range %		87-98

回收率

The recovery of human KEAP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		103		95-107
EDTA plasma (n=4)		88		83-94

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected 1600 2.968 2.856 2.912 2.777 800 2.121 2.222 2.172 2.037 400 1.423 1.485 1.454 1.319 200 0.864 0.847 0.856 0.721 100 0.553 0.537 0.545 0.410 50 0.372 0.384 0.378 0.243 25 0.242 0.247 0.245 0.110 0 0.136 0.134 0.135

货期

3-5 working days