产品描述
IL-35(干扰素样21,IFN-λ5)是一种抑制性的细胞因子,属于IL-12家族。它由EBV转化的B细胞、Breg和Treg产生,具有调节和抑制细胞免疫功能的作用。IL-35被认为是抑制T细胞和B细胞活化的重要细胞因子,可以抑制Th17细胞的分化和功能。IL-35在某些自身免疫疾病和肿瘤的免疫治疗中显示出一定的治疗前景。
华美生物所提供的Human Interleukin 35(IL-35) ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、细胞培养上清、尿液、组织匀浆样本中的IL-35,其灵敏度为15.6 pg/mL,检测范围为62.5 pg/mL-4000 pg/mL。
缩写
IL-35
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL-35 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
94 |
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| Range % |
90-98 |
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| 1:2 |
Average % |
98 |
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| Range % |
94-103 |
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| 1:4 |
Average % |
97 |
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| Range % |
90-106 |
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| 1:8 |
Average % |
94 |
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| Range % |
88-109 |
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回收率
| The recovery of human IL-35 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
87-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.872 |
2.752 |
2.812 |
2.705 |
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| 2000 |
2.279 |
2.179 |
2.229 |
2.122 |
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| 1000 |
1.545 |
1.589 |
1.567 |
1.460 |
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| 500 |
0.952 |
1.002 |
0.977 |
0.870 |
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| 250 |
0.531 |
0.575 |
0.553 |
0.446 |
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| 125 |
0.341 |
0.366 |
0.354 |
0.247 |
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| 62.5 |
0.255 |
0.275 |
0.265 |
0.158 |
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| 0 |
0.108 |
0.105 |
0.107 |
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货期
3-5 working days