产品描述
This Mouse Myoglobin(MB) ELISA Kit was designed for the quantitative measurement of Mouse Myoglobin(MB) protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL.
别名
Mb ELISA Kit; Myoglobin ELISA Kit
缩写
MB
Uniprot No
P04247
灵敏度
3.9 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse MYO/MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 83-94 | | |
| 1:2 | Average % | 98 | | |
| Range % | 94-101 | | |
| 1:4 | Average % | 102 | | |
| Range % | 98-105 | | |
| 1:8 | Average % | 95 | | |
| Range % | 90-101 | | |
回收率
| The recovery of mouse MYO/MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
|
| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 91 | 85-105 | | |
| EDTA plasma (n=4) | 104 | 88-109 | | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | | 1000 | 2.511 | 2.622 | 2.567 | 2.387 | | | | 500 | 2.106 | 2.121 | 2.114 | 1.934 | | | | 250 | 1.715 | 1.723 | 1.719 | 1.539 | | | | 125 | 1.177 | 1.232 | 1.205 | 1.025 | | | | 62.5 | 0.809 | 0.821 | 0.815 | 0.635 | | | | 31.2 | 0.544 | 0.524 | 0.534 | 0.354 | | | | 15.6 | 0.372 | 0.377 | 0.375 | 0.195 | | | | 0 | 0.179 | 0.181 | 0.180 | | | |
|
货期
3-5 working days