产品描述
This Goat IL1B ELISA Kit was designed for the quantitative measurement of Goat IL1B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.
别名
IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写
IL-1β
Uniprot No
P79162
灵敏度
0.78 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 82-94 | | |
| 1:2 | Average % | 90 | | |
| Range % | 82-97 | | |
| 1:4 | Average % | 92 | | |
| Range % | 85-100 | | |
| 1:8 | Average % | 95 | | |
| Range % | 90-103 | | |
回收率
| The recovery of goat IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 90 | 85-98 | | |
| EDTA plasma (n=4) | 99 | 93-108 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| pg/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 2.112 | 2.052 | 2.082 | 1.951 | | | | 100 | 1.576 | 1.654 | 1.615 | 1.484 | | | | 50 | 1.039 | 0.984 | 1.012 | 0.881 | | | | 25 | 0.587 | 0.549 | 0.568 | 0.437 | | | | 12.5 | 0.384 | 0.397 | 0.391 | 0.260 | | | | 6.25 | 0.292 | 0.269 | 0.281 | 0.150 | | | | 3.12 | 0.208 | 0.199 | 0.204 | 0.073 | | | | 0 | 0.127 | 0.134 | 0.131 | | | |
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货期
3-5 working days