产品描述

This Human KRAS ELISA Kit was designed for the quantitative measurement of Human KRAS protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

c Ki ras2 ELISA Kit; c Kirsten ras protein ELISA Kit; c-K-ras ELISA Kit; c-Ki-ras ELISA Kit; Cellular c Ki ras2 proto oncogene ELISA Kit; Cellular transforming proto oncogene ELISA Kit; CFC2 ELISA Kit; cK Ras ELISA Kit; GTPase KRas ELISA Kit; K RAS p21 protein ELISA Kit; K RAS2A ELISA Kit; K RAS2B ELISA Kit; K RAS4A ELISA Kit; K RAS4B ELISA Kit; K-Ras 2 ELISA Kit; KI RAS ELISA Kit; Ki-Ras ELISA Kit; KIRSTEN MURINE SARCOMA VIRUS 2 ELISA Kit; Kirsten rat sarcoma 2 viral (v Ki ras2) oncogene homolog ELISA Kit; Kirsten rat sarcoma viral oncogene homolog ELISA Kit; KRAS ELISA Kit; KRAS proto oncogene, GTPase ELISA Kit; KRAS1 ELISA Kit; KRAS2 ELISA Kit; N-terminally processed ELISA Kit; NS ELISA Kit; NS3 ELISA Kit; Oncogene KRAS2 ELISA Kit; p21ras ELISA Kit; PR310 c K ras oncogene ELISA Kit; PR310 cK ras oncogene ELISA Kit; RALD ELISA Kit; RASK_HUMAN ELISA Kit; RASK2 ELISA Kit; Transforming protein p21 ELISA Kit; v Ki ras2 Kirsten rat sarcoma 2 viral oncogene homolog ELISA Kit; v Ki ras2 Kirsten rat sarcoma viral oncogene homolog ELISA Kit

缩写

KRAS

Uniprot No

P01116

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KRAS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		92
		Range %		86-102
1:2		Average %		92
		Range %		86-108
1:4		Average %		89
		Range %		82-98
1:8		Average %		95
		Range %		88-100

回收率

The recovery of human KRAS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		90-97
EDTA plasma (n=4)		88		83-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 1.756 1.814 1.785 1.631 750 1.062 1.123 1.093 0.939 375 0.638 0.629 0.634 0.480 187.5 0.427 0.422 0.425 0.271 94 0.303 0.317 0.310 0.156 47 0.254 0.263 0.259 0.105 23.5 0.196 0.201 0.199 0.045 0 0.151 0.156 0.154

货期

3-5 working days