产品描述
This Rat MPO-ANCA ELISA Kit was designed for the quantitative measurement of Rat MPO-ANCA protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.625 U/mL-40 U/mL and the sensitivity is 0.156 U/mL .
别名
N/A
缩写
MPO-ANCA
灵敏度
0.156 U/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MPO-ANCA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-95 | |
| 1:2 | Average % | 97 | |
| Range % | 91-105 | |
| 1:4 | Average % | 96 | |
| Range % | 92-100 | |
| 1:8 | Average % | 93 | |
| Range % | 85-98 | |
回收率
| The recovery of rat MPO-ANCA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-97 | |
| EDTA plasma (n=4) | 91 | 84-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| U/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.612 | 2.752 | 2.682 | 2.518 | | | 20 | 2.013 | 2.076 | 2.045 | 1.881 | | | 10 | 1.367 | 1.304 | 1.336 | 1.172 | | | 5 | 0.802 | 0.837 | 0.820 | 0.656 | | | 2.5 | 0.444 | 0.424 | 0.434 | 0.270 | | | 1.25 | 0.351 | 0.359 | 0.355 | 0.191 | | | 0.625 | 0.241 | 0.225 | 0.233 | 0.069 | | | 0 | 0.160 | 0.167 | 0.164 | | |
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货期
3-5 working days