产品描述
This Human NAPSA ELISA Kit was designed for the quantitative measurement of Human NAPSA protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 4.7 ng/mL-300 ng/mL and the sensitivity is 1.18 ng/mL.
别名
Asp 4 ELISA Kit; ASP4 ELISA Kit; Aspartyl protease 4 ELISA Kit; KAP ELISA Kit; Kdap ELISA Kit; Kidney derived aspartic protease like protein ELISA Kit; NAP1 ELISA Kit; NAPA ELISA Kit; Napsa ELISA Kit; NAPSA_HUMAN ELISA Kit; Napsin 1 ELISA Kit; napsin A aspartic peptidase ELISA Kit; Napsin A precursor ELISA Kit; Napsin-1 ELISA Kit; Napsin-A ELISA Kit; Pronapsin A ELISA Kit; SNAPA ELISA Kit; TA01/TA02 ELISA Kit
缩写
NAPSA
Uniprot No
O96009
灵敏度
1.18 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human NAPSA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:5 | Average % | 104 | | |
| Range % | 97-108 | | |
| 1:10 | Average % | 101 | | |
| Range % | 95-104 | | |
| 1:20 | Average % | 97 | | |
| Range % | 89-101 | | |
| 1:40 | Average % | 106 | | |
| Range % | 98-110 | | |
回收率
| The recovery of human NAPSA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 101 | 95-104 | | |
| EDTA plasma (n=4) | 101 | 94-105 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 300 | 2.435 | 2.527 | 2.481 | 2.289 | | | | 150 | 2.042 | 2.104 | 2.073 | 1.881 | | | | 75 | 1.455 | 1.409 | 1.432 | 1.240 | | | | 37.5 | 0.843 | 0.823 | 0.833 | 0.641 | | | | 18.75 | 0.552 | 0.538 | 0.545 | 0.353 | | | | 9.4 | 0.381 | 0.376 | 0.379 | 0.187 | | | | 4.7 | 0.301 | 0.311 | 0.306 | 0.114 | | | | 0 | 0.190 | 0.194 | 0.192 | | | |
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货期
3-5 working days