产品描述
The Human Oncostatin-M (OSM) ELISA KIT is an accurate and reliable tool for the detection of Oncostatin-M protein levels in human serum, plasma, and cell culture supernates. Oncostatin-M is a multifunctional cytokine that plays a critical role in cell growth,immune response regulation and inflammation.
The Human OSM ELISA KIT has a detection range of 6.25 pg/mL-400 pg/mL and a high sensitivity of 1.56 pg/mL, ensuring precise and accurate measurements even at low concentrations. The kit uses a sandwich assay principle, which involves the use of two monoclonal antibodies specific to the human OSM protein.
The assay time for the kit is 1-5 hours, and the sample volume required is 50-100ul. Detection is done at a wavelength of 450 nm, making it easy to read and interpret the results. The kit is suitable for use in immunology and cell research, where OSM protein levels are of interest.
别名
MGC20461 ELISA Kit; ONCM_HUMAN ELISA Kit; Oncostatin M ELISA Kit; Oncostatin-M ELISA Kit; OSM ELISA Kit
缩写
OSM
Uniprot No
P13725
灵敏度
1.56 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human OSM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
83-97 |
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| 1:2 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:4 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:8 |
Average % |
89 |
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| Range % |
81-95 |
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回收率
| The recovery of human OSM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
101 |
94-102 |
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| EDTA plasma (n=4) |
107 |
100-115 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
1.779 |
1.844 |
1.812 |
1.642 |
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| 200 |
1.011 |
1.023 |
1.017 |
0.847 |
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| 100 |
0.685 |
0.666 |
0.676 |
0.506 |
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| 50 |
0.421 |
0.415 |
0.418 |
0.248 |
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| 25 |
0.296 |
0.302 |
0.299 |
0.129 |
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| 12.5 |
0.251 |
0.265 |
0.258 |
0.088 |
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| 6.25 |
0.222 |
0.232 |
0.227 |
0.057 |
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| 0 |
0.165 |
0.174 |
0.170 |
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货期
3-5 working days
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