产品描述

This Human PAPPA ELISA Kit was designed for the quantitative measurement of Human PAPPA protein in serum, plasma, urine. It is a Sandwich ELISA kit, its detection range is 1.7 ng/mL-50 ng/mL and the sensitivity is 0.4 ng/mL.

别名

ASBABP2 ELISA Kit; Aspecific BCL2 ARE binding protein 2 ELISA Kit; Differentially placenta 1 expressed protein ELISA Kit; DIPLA1 ELISA Kit; EC 3.4.24.79 ELISA Kit; IGF dependent IGFBP 4 protease ELISA Kit; IGF-dependent IGFBP-4 protease ELISA Kit; IGFBP 4ase ELISA Kit; IGFBP-4ase ELISA Kit; IGFBP4ase ELISA Kit; Insulin like growth factor dependent IGF binding protein 4 protease ELISA Kit; Insulin-like growth factor-dependent IGF-binding protein 4 protease ELISA Kit; PAPA ELISA Kit; PAPP A ELISA Kit; PAPP-A ELISA Kit; PAPP1_HUMAN ELISA Kit; PAPPA ELISA Kit; PAPPA1 ELISA Kit; Pappalysin 1 precursor ELISA Kit; Pappalysin-1 ELISA Kit; Pregnancy Associated Plasma Protein A ELISA Kit; Pregnancy associated plasma protein A pappalysin 1 ELISA Kit; Pregnancy-associated plasma protein A ELISA Kit

缩写

PAPPA

Uniprot No

Q13219

灵敏度

0.4 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human PAPP-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:5		Average %		86
		Range %		80-93
1:10		Average %		90
		Range %		85-95
1:20		Average %		95
		Range %		90-100
1:40		Average %		94
		Range %		86-100

回收率

The recovery of human PAPP-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		95		89-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   50 2.542 2.452 2.497 2.450   20.8 1.283 1.356 1.320 1.273   8.3 0.642 0.631 0.637 0.590   3.3 0.287 0.305 0.296 0.249   1.7 0.155 0.160 0.158 0.111   0 0.046 0.047 0.047

货期

3-5 working days