产品描述

This Human LMNA ELISA Kit was designed for the quantitative measurement of Human LMNA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.

别名

70 kDa lamin ELISA Kit; Cardiomyopathy dilated 1A (autosomal dominant) ELISA Kit; CDCD1 ELISA Kit; CDDC ELISA Kit; CMD1A ELISA Kit; CMT2B1 ELISA Kit; EMD2 ELISA Kit; FPL ELISA Kit; FPLD ELISA Kit; FPLD2 ELISA Kit; HGPS ELISA Kit; IDC ELISA Kit; Lamin A ELISA Kit; Lamin A/C ELISA Kit; Lamin A/C like 1 ELISA Kit; Lamin ELISA Kit; Lamin C ELISA Kit; lamin-a ELISA Kit; Lamin-A/C ELISA Kit; LDP1 ELISA Kit; LFP ELISA Kit; LGMD1B ELISA Kit; Limb girdle muscular dystrophy 1B (autosomal dominant) ELISA Kit; LMN 1 ELISA Kit; LMN A ELISA Kit; LMN C ELISA Kit; LMN1 ELISA Kit; LMNA ELISA Kit; LMNA_HUMAN ELISA Kit; LMNC ELISA Kit; LMNL1 ELISA Kit; Prelamin A/C ELISA Kit; PRO1 ELISA Kit; Renal carcinoma antigen NY REN 32 ELISA Kit; Renal carcinoma antigen NY-REN-32 ELISA Kit; Renal carcinoma antigen NYREN32 ELISA Kit

缩写

LMNA

Uniprot No

P02545

灵敏度

3.9 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LMNA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		88
		Range %		85-91
1:2		Average %		103
		Range %		100-106
1:4		Average %		85
		Range %		82-89
1:8		Average %		96
		Range %		93-99

回收率

The recovery of human LMNA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		84		81-87
EDTA plasma (n=4)		94		91-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1000 2.434 2.468 2.451 2.302 500 1.661 1.698 1.680 1.531 250 1.094 1.072 1.083 0.934 125 0.656 0.627 0.642 0.493 62.5 0.426 0.442 0.434 0.285 31.2 0.309 0.318 0.314 0.165 15.6 0.258 0.248 0.253 0.104 0 0.150 0.148 0.149

货期

3-5 working days