产品描述
CUSABIO’s Human lipopolysaccharide-binding protein (LBP) ELISA Kit is suitable for the quantitative detection of LBP content in human serum, plasma, or tissue homogenates. LBP is a carrier protein of the endotoxins' recognition by the host. It is produced by hepatocytes and released into the bloodstream as a consequence of Gram-negative infection. LBP conveys LPS to the cellular surface, forming a ternary complex with a cluster of CD14. CD14 facilitates the transfer of LPS to the TLR4/MD2 complex, activating LPS-TLR4 signaling that elicits proinflammatory responses. It is significantly up-regulated during acute-phase reaction following the initial inflammatory responses, promoting, along with (bactericidal/permeability-increasing protein), non-inflammatory clearance of LPS, and ultimate resolution of LPS-induced inflammation. Additionally, LBP mediates endothelial dysfunction and is required for apoptosis in tubular cells.
The detection mechanism of this kit is based on the sandwich ELISA technique and enzyme-substrate chromogenic reaction. The formed pre-coated LBP antibody/LBP/Biotin-conjugated LBP antibody complex is labeled by HRP-avidin and then develops a color reaction after the addition of The TMB substrate solution. The color intensity can be measured at 450 nm via a microplate reader.
别名
BPI fold containing family D, member 2 ELISA Kit; Bpifd2 ELISA Kit; LBP ELISA Kit; LBP_HUMAN ELISA Kit; LBP1 ELISA Kit; Lipopolysaccharide binding protein ELISA Kit; Lipopolysaccharide-binding protein ELISA Kit; LPS binding protein ELISA Kit; Ly88 ELISA Kit; MGC22233 ELISA Kit; OTTHUMP00000030965 ELISA Kit; RP23-407H16.4 ELISA Kit
缩写
LBP
Uniprot No
P18428
灵敏度
0.156 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human LBP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 97 | |
| Range % | 92-102 | |
| 1:2 | Average % | 95 | |
| Range % | 91-99 | |
| 1:4 | Average % | 99 | |
| Range % | 96-108 | |
| 1:8 | Average % | 94 | |
| Range % | 89-98 | |
回收率
| The recovery of human LBP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 99 | 92-108 | |
| EDTA plasma (n=4) | 84 | 80-88 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
 | μg/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.795 | 2.694 | 2.745 | 2.613 | | | 20 | 2.232 | 2.131 | 2.182 | 2.050 | | | 10 | 1.464 | 1.443 | 1.454 | 1.322 | | | 5 | 0.812 | 0.823 | 0.818 | 0.686 | | | 2.5 | 0.425 | 0.415 | 0.420 | 0.288 | | | 1.25 | 0.300 | 0.311 | 0.306 | 0.174 | | | 0.625 | 0.222 | 0.218 | 0.220 | 0.088 | | | 0 | 0.132 | 0.131 | 0.132 | | |
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货期
3-5 working days
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