产品描述
This Pig NT-proBNP ELISA Kit was designed for the quantitative measurement of Pig NT-proBNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL.
缩写
NT-proBNP
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cardiovascular
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of pig NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 103 | |
| Range % | 96-108 | |
| 1:2 | Average % | 99 | |
| Range % | 91-102 | |
| 1:4 | Average % | 94 | |
| Range % | 86-98 | |
| 1:8 | Average % | 98 | |
| Range % | 90-102 | |
回收率
| The recovery of pig NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 105 | 99-109 | |
| EDTA plasma (n=4) | 97 | 90-101 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.129 | 2.045 | 2.087 | 1.949 | | | 250 | 1.671 | 1.555 | 1.613 | 1.475 | | | 125 | 1.134 | 1.117 | 1.126 | 0.988 | | | 62.5 | 0.666 | 0.650 | 0.658 | 0.520 | | | 31.2 | 0.388 | 0.371 | 0.380 | 0.242 | | | 15.6 | 0.257 | 0.266 | 0.262 | 0.124 | | | 7.8 | 0.171 | 0.167 | 0.169 | 0.031 | | | 0 | 0.137 | 0.139 | 0.138 | | |
|
货期
3-5 working days