产品描述
This Goat IL6 ELISA Kit was designed for the quantitative measurement of Goat IL6 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.
别名
IL6Interleukin-6 ELISA Kit; IL-6 ELISA Kit
缩写
IL6
Uniprot No
Q28319
灵敏度
0.39 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of goat IL-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 85 | |
| Range % | 80-91 | |
| 1:2 | Average % | 102 | |
| Range % | 97-107 | |
| 1:4 | Average % | 84 | |
| Range % | 81-89 | |
| 1:8 | Average % | 99 | |
| Range % | 96-104 | |
回收率
| The recovery of goat IL-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 90-101 | |
| EDTA plasma (n=4) | 102 | 97-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.625 | 2.698 | 2.662 | 2.542 | | | 50 | 1.959 | 2.001 | 1.980 | 1.860 | | | 25 | 1.221 | 1.314 | 1.268 | 1.148 | | | 12.5 | 0.741 | 0.705 | 0.723 | 0.603 | | | 6.25 | 0.455 | 0.468 | 0.462 | 0.342 | | | 3.12 | 0.307 | 0.318 | 0.313 | 0.193 | | | 1.56 | 0.238 | 0.237 | 0.238 | 0.118 | | | 0 | 0.121 | 0.119 | 0.120 | | |
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货期
3-5 working days