产品描述

This human LRP1 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human LRP1 in multiple samples, including serum, plasma, cell lysates, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the LRP1 levels in the sample and is measured at 450 nm through a microplate reader.

LRP1 is involved in two major physiological processes: endocytosis and regulation of signaling pathways, which play an important role in multiple biological activities, including regulation of cell growth and differentiation, modulation of cytoskeletal organization angiogenesis, cell migration, and regulation of protease degradation for tissue invasion. LRP1 mediates the endocytosis of a diverse set of extracellular ligands that play important roles in tumor progression. Additionally, LRP1 can initiate and regulate diverse signaling pathways, including MAPK, insulin receptor (IR), serine/AKT, ERK, and JNK pathways. LRP1 has been postulated to participate in numerous diverse physiological and pathological processes ranging from plasma lipoprotein homeostasis, atherosclerosis, tumor evolution, and fibrinolysis to neuronal regeneration and survival.

别名

A2MR ELISA Kit; Alpha 2 macroglobulin receptor ELISA Kit; alpha 2MR ELISA Kit; Alpha-2-macroglobulin receptor ELISA Kit; APOER ELISA Kit; Apolipoprotein E receptor ELISA Kit; APR ELISA Kit; CD 91 ELISA Kit; CD91 ELISA Kit; CD91 antigen ELISA Kit; IGFBP3R ELISA Kit; LDL receptor related protein 1 ELISA Kit; Low density lipoprotein receptor related protein 1 ELISA Kit; Low density lipoprotein related protein 1 ELISA Kit; Low-density lipoprotein receptor-related protein 1 intracellular domain ELISA Kit; LRP 1 ELISA Kit; LRP 515 ELISA Kit; LRP 85 ELISA Kit; LRP ELISA Kit; LRP ICD ELISA Kit; LRP-1 ELISA Kit; LRP-515 ELISA Kit; LRP-85 ELISA Kit; Lrp1 ELISA Kit; LRP1 protein ELISA Kit; LRP1_HUMAN ELISA Kit; LRP1A ELISA Kit; LRP515 ELISA Kit; LRP85 ELISA Kit; LRPICD ELISA Kit; MGC88725 ELISA Kit; Prolow density lipoprotein receptor related protein 1 ELISA Kit; TbetaR V/LRP 1/IGFBP 3 receptor ELISA Kit; TbetaRV/LRP1/IGFBP3 receptor ELISA Kit; TGFBR 5 ELISA Kit; TGFBR5 ELISA Kit; Type V tgf beta receptor ELISA Kit

缩写

LRP1

Uniprot No

Q07954

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LRP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		93
		Range %		88-97
1:2		Average %		84
		Range %		80-89
1:4		Average %		102
		Range %		96-108
1:8		Average %		97
		Range %		91-102

回收率

The recovery of human LRP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		86		82-94
EDTA plasma (n=4)		99		92-107

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 40 2.455 2.354 2.405 2.262 20 1.628 1.527 1.578 1.435 10 0.976 0.955 0.966 0.823 5 0.566 0.577 0.572 0.429 2.5 0.371 0.382 0.377 0.234 1.25 0.267 0.278 0.273 0.130 0.625 0.210 0.216 0.213 0.070 0 0.142 0.143 0.143

货期

3-5 working days