产品描述
This Rat IL-22 ELISA Kit was designed for the quantitative measurement of Rat IL-22 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
缩写
IL-22
Uniprot No
G3V6X6
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
| 1:1 | Average % | 88 | |
| Range % | 83-94 | |
| 1:2 | Average % | 99 | |
| Range % | 90-102 | |
| 1:4 | Average % | 93 | |
| Range % | 85-97 | |
| 1:8 | Average % | 92 | |
| Range % | 84-96 | |
回收率
| The recovery of rat IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 101 | 95-105 | |
| EDTA plasma (n=4) | 104 | 92-109 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.120 | 2.147 | 2.134 | 1.958 | | | 500 | 1.493 | 1.486 | 1.490 | 1.314 | | | 250 | 1.029 | 1.105 | 1.067 | 0.891 | | | 125 | 0.658 | 0.673 | 0.666 | 0.490 | | | 62.5 | 0.456 | 0.444 | 0.450 | 0.274 | | | 31.2 | 0.346 | 0.358 | 0.352 | 0.176 | | | 15.6 | 0.270 | 0.262 | 0.266 | 0.090 | | | 0 | 0.174 | 0.178 | 0.176 | | |
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货期
3-5 working days