产品描述
This Human OX-B ELISA Kit was designed for the quantitative measurement of Human OX-B protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 62.5 pg/mL-4000 pg/mL and the sensitivity is 15.6 pg/mL.
缩写
OX-B
灵敏度
15.6 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Metabolism
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays):CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human OX-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
107 |
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| Range % |
95-111 |
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| 1:2 |
Average % |
100 |
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| Range % |
93-104 |
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| 1:4 |
Average % |
94 |
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| Range % |
86-97 |
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| 1:8 |
Average % |
100 |
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| Range % |
92-104 |
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回收率
| The recovery of human OX-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
91 |
84-95 |
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| EDTA plasma (n=4) |
108 |
97-112 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.312 |
2.378 |
2.345 |
2.212 |
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| 2000 |
1.386 |
1.346 |
1.366 |
1.233 |
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| 1000 |
0.820 |
0.812 |
0.816 |
0.683 |
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| 500 |
0.523 |
0.504 |
0.514 |
0.381 |
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| 250 |
0.370 |
0.356 |
0.363 |
0.230 |
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| 125 |
0.278 |
0.268 |
0.273 |
0.140 |
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| 62.5 |
0.209 |
0.203 |
0.206 |
0.073 |
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| 0 |
0.132 |
0.134 |
0.133 |
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货期
3-5 working days