产品描述
This Human NEFL ELISA Kit was designed for the quantitative measurement of Human NEFL protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
NEFL; NF68; NFL; Neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; Neurofilament triplet L protein
缩写
NEFL
Uniprot No
P07196
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Neuroscience
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human NF-L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 99 | |
| Range % | 91-105 | |
| 1:10 | Average % | 85 | |
| Range % | 80-92 | |
| 1:20 | Average % | 88 | |
| Range % | 82-94 | |
| 1:40 | Average % | 93 | |
| Range % | 86-98 | |
回收率
| The recovery of human NF-L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 89-98 | |
| EDTA plasma (n=4) | 98 | 92-104 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.770 | 2.665 | 2.718 | 2.628 | | | 1000 | 2.114 | 2.006 | 2.060 | 1.970 | | | 500 | 1.256 | 1.243 | 1.250 | 1.160 | | | 250 | 0.657 | 0.651 | 0.654 | 0.564 | | | 125 | 0.343 | 0.339 | 0.341 | 0.251 | | | 62.5 | 0.252 | 0.242 | 0.247 | 0.157 | | | 31.25 | 0.209 | 0.198 | 0.204 | 0.114 | | | 0 | 0.092 | 0.088 | 0.090 | | |
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货期
3-5 working days