产品描述
This Rabbit IL2 ELISA Kit was designed for the quantitative measurement of Rabbit IL2 protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.8 pg/mL.
别名
IL2Interleukin-2 ELISA Kit; IL-2 ELISA Kit; T-cell growth factor ELISA Kit; TCGF ELISA Kit
缩写
IL2
Uniprot No
O77620
灵敏度
7.8 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 87 | |
| Range % | 82-93 | |
| 1:2 | Average % | 95 | |
| Range % | 91-100 | |
| 1:4 | Average % | 86 | |
| Range % | 80-95 | |
| 1:8 | Average % | 93 | |
| Range % | 82-98 | |
回收率
| The recovery of rabbit IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 80-98 | |
| EDTA plasma (n=4) | 93 | 84-100 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.412 | 2.320 | 2.366 | 2.181 | | | 1000 | 2.174 | 2.081 | 2.128 | 1.943 | | | 500 | 1.882 | 1.743 | 1.813 | 1.628 | | | 250 | 1.521 | 1.482 | 1.502 | 1.317 | | | 125 | 1.048 | 1.044 | 1.046 | 0.861 | | | 62.5 | 0.688 | 0.654 | 0.671 | 0.486 | | | 31.25 | 0.331 | 0.308 | 0.320 | 0.135 | | | 0 | 0.191 | 0.179 | 0.185 | | |
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货期
3-5 working days