产品描述
IgG是一种免疫球蛋白,广泛存在于人体的血浆和组织液中,占所有免疫球蛋白中数量最多的一种。它有很多生物学功能,比如保护机体免受感染、清除异物和毒素、识别和结合病原体等。此外,IgG还参与许多免疫反应的调节,比如激活补体、介导细胞介导的毒性和抗体依赖性细胞介导的细胞毒性等。因此,IgG的检测对于疾病的诊断和治疗具有重要意义。
华美生物所提供的Rat Immunoglobulin G,IgG ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测大鼠血清、血浆样本中的IgG,其灵敏度为31.25 ng/mL,检测范围为31.25 ng/mL-8000 ng/mL。
别名
N/A
缩写
IgG
灵敏度
31.25 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat IgG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
100 |
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| Range % |
95-104 |
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| 1:2000 |
Average % |
90 |
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| Range % |
86-95 |
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| 1:4000 |
Average % |
87 |
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| Range % |
83-92 |
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| 1:8000 |
Average % |
101 |
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| Range % |
97-106 |
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回收率
| The recovery of rat IgG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
88-95 |
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| EDTA plasma (n=4) |
93 |
89-97 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
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| 8000 |
0.230 |
0.225 |
0.228 |
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| 2000 |
0.417 |
0.407 |
0.412 |
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| 500 |
0.643 |
0.664 |
0.654 |
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| 125 |
1.238 |
1.257 |
1.248 |
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| 31.25 |
1.754 |
1.770 |
1.762 |
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| 0 |
2.035 |
1.988 |
2.012 |
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货期
3-5 working days