产品描述

This Human ITIH1 ELISA Kit was designed for the quantitative measurement of Human ITIH1 protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 ng/mL-1000 ng/mL and the sensitivity is 3.9 ng/mL .

别名

H1P ELISA Kit; IATIH ELISA Kit; IGHEP1 ELISA Kit; Inter alpha (globulin) inhibitor H1 ELISA Kit; Inter alpha (globulin) inhibitor H1 polypeptide ELISA Kit; Inter alpha trypsin inhibitor heavy chain 1 ELISA Kit; Inter alpha trypsin inhibitor heavy chain H1 ELISA Kit; Inter-alpha-inhibitor heavy chain 1 ELISA Kit; Inter-alpha-trypsin inhibitor complex component III ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H1 ELISA Kit; ITI heavy chain H1 ELISA Kit; ITI-HC1 ELISA Kit; ITIH ELISA Kit; ITIH1 ELISA Kit; ITIH1_HUMAN ELISA Kit; Serum-derived hyaluronan-associated protein ELISA Kit; SHAP ELISA Kit

缩写

ITIH1

Uniprot No

P19827

灵敏度

3.9 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		86
		Range %		80-92
1:200		Average %		97
		Range %		91-105
1:400		Average %		99
		Range %		92-110
1:800		Average %		97
		Range %		86-98

回收率

The recovery of human ITIH1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-98
EDTA plasma (n=4)		97		90-104

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected 1000 2.400 2.418 2.409 2.328 500 2.088 1.999 2.004 1.923 250 1.562 1.555 1.559 1.478 125 1.228 1.211 1.220 1.139 62.5 0.689 0.665 0.677 0.596 31.25 0.452 0.436 0.444 0.363 15.6 0.270 0.254 0.262 0.181 0 0.084 0.078 0.081

货期

3-5 working days