产品描述

This Human IMPDH2 ELISA Kit was designed for the quantitative measurement of Human IMPDH2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 23.5 pg/mL-1500 pg/mL and the sensitivity is 5.8 pg/mL.

别名

IMDH2_HUMAN ELISA Kit; IMP (inosine monophosphate) dehydrogenase 2 ELISA Kit; IMP dehydrogenase 2 ELISA Kit; IMP oxireductase 2 ELISA Kit; IMPD 2 ELISA Kit; IMPD2 ELISA Kit; IMPDH 2 ELISA Kit; IMPDH II ELISA Kit; IMPDH-II ELISA Kit; Impdh2 ELISA Kit; Inosine 5' monophosphate dehydrogenase 2 ELISA Kit; Inosine 5' phosphate dehydrogenase 2 ELISA Kit; Inosine monophosphate dehydrogenase type II ELISA Kit; Inosine-5''-monophosphate dehydrogenase 2 ELISA Kit

缩写

IMPDH2

Uniprot No

P12268

灵敏度

5.8 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human IMPDH2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		94
		Range %		88-98
1:2		Average %		97
		Range %		93-102
1:4		Average %		94
		Range %		89-98
1:8		Average %		87
		Range %		82-91

回收率

The recovery of human IMPDH2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		98		94-102
EDTA plasma (n=4)		88		82-92

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 1500 2.632 2.510 2.571 2.448 750 1.476 1.519 1.498 1.375 375 0.867 0.820 0.844 0.721 187.5 0.473 0.465 0.469 0.346 94 0.305 0.311 0.308 0.185 47 0.258 0.246 0.252 0.129 23.5 0.189 0.185 0.187 0.064 0 0.124 0.122 0.123

货期

3-5 working days