产品描述
This Rat Nesfatin-1 ELISA Kit was designed for the quantitative measurement of Rat Nesfatin-1 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL .
缩写
Nesfatin-1
灵敏度
3.9 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
| | | | | | | |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat nesfatin-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 84 | |
| Range % | 80-92 | |
| 1:2 | Average % | 89 | |
| Range % | 85-95 | |
| 1:4 | Average % | 93 | |
| Range % | 89-99 | |
| 1:8 | Average % | 95 | |
| Range % | 90-100 | |
回收率
| The recovery of rat nesfatin-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 104 | 97-109 | |
| EDTA plasma (n=4) | 97 | 93-102 | |
| | | | | | | |
| | | | | | | |
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 1000 | 2.795 | 2.652 | 2.724 | 2.573 | | | 500 | 1.857 | 1.776 | 1.817 | 1.666 | | | 250 | 1.004 | 1.082 | 1.043 | 0.892 | | | 125 | 0.654 | 0.613 | 0.634 | 0.483 | | | 62.5 | 0.424 | 0.430 | 0.427 | 0.276 | | | 31.2 | 0.317 | 0.299 | 0.308 | 0.157 | | | 15.6 | 0.226 | 0.209 | 0.218 | 0.067 | | | 0 | 0.154 | 0.147 | 0.151 | | |
|
货期
3-5 working days