产品描述
IL1B (白细胞介素-1β,Interleukin-1β) 是由许多不同类型的细胞产生的促炎症细胞因子,包括单核细胞、巨噬细胞、树突状细胞、T细胞和B细胞等。IL1B 参与了许多生理和病理过程,包括炎症、感染、肿瘤、自身免疫疾病等。
华美生物所提供的Mouse Interleukin 1β,IL-1β ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测鼠血清、血浆、细胞培养上清、组织匀浆样本中的IL1B,其灵敏度为7.8 pg/mL,检测范围为31.25 pg/mL-2000 pg/mL。
别名
Interleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写
IL1B
Uniprot No
P10749
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<10% |
|
| Three samples of known concentration were tested in twenty assays to assess. |
线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
88 |
|
| |
Range % |
82-95 |
|
| 1:2 |
Average % |
93 |
|
| |
Range % |
88-98 |
|
| 1:4 |
Average % |
96 |
|
| |
Range % |
94-102 |
|
| 1:8 |
Average % |
94 |
|
| |
Range % |
90-100 |
|
回收率
| The recovery of mouse IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
87-99 |
|
| EDTA plasma (n=4) |
93 |
88-98 |
|
标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
|
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 2000 |
2.758 |
2.641 |
2.700 |
2.586 |
|
| 1000 |
1.438 |
1.587 |
1.513 |
1.399 |
|
| 500 |
0.833 |
0.869 |
0.851 |
0.737 |
|
| 250 |
0.474 |
0.477 |
0.476 |
0.362 |
|
| 125 |
0.350 |
0.375 |
0.363 |
0.249 |
|
| 62.5 |
0.267 |
0.253 |
0.260 |
0.146 |
|
| 31.25 |
0.165 |
0.186 |
0.176 |
0.062 |
|
| 0 |
0.119 |
0.108 |
0.114 |
|
|
货期
3-5 working days