产品描述
This Human IL17F ELISA Kit was designed for the quantitative measurement of Human IL17F protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.1 pg/mL .
别名
CANDF6 ELISA Kit; Cytokine ML 1 ELISA Kit; Cytokine ML-1 ELISA Kit; IL 17F ELISA Kit; IL 24 ELISA Kit; IL-17F ELISA Kit; IL-24 ELISA Kit; Il17f ELISA Kit; IL17F_HUMAN ELISA Kit; Interleukin 17F ELISA Kit; Interleukin 24 ELISA Kit; Interleukin-17F ELISA Kit; Interleukin-24 ELISA Kit; ML 1 ELISA Kit; ML1 ELISA Kit; Mutant IL 17F ELISA Kit; OTTHUMP00000016602 ELISA Kit
缩写
IL17F
Uniprot No
Q96PD4
灵敏度
3.1 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IL17F in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 93 | |
| Range % | 87-99 | |
| 1:2 | Average % | 103 | |
| Range % | 99-108 | |
| 1:4 | Average % | 80 | |
| Range % | 85-96 | |
| 1:8 | Average % | 93 | |
| Range % | 85-98 | |
回收率
| The recovery of human IL17F spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 92 | 87-96 | |
| EDTA plasma (n=4) | 89 | 85-93 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 800 | 1.850 | 1.920 | 1.885 | 1.795 | | | 400 | 1.342 | 1.405 | 1.374 | 1.284 | | | 200 | 0.834 | 0.902 | 0.868 | 0.778 | | | 100 | 0.557 | 0.525 | 0.541 | 0.451 | | | 50 | 0.325 | 0.342 | 0.334 | 0.244 | | | 25 | 0.253 | 0.264 | 0.259 | 0.169 | | | 12.5 | 0.146 | 0.150 | 0.148 | 0.058 | | | 0 | 0.087 | 0.092 | 0.090 | | |
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货期
3-5 working days