产品描述

This Human ITIH4 ELISA Kit was designed for the quantitative measurement of Human ITIH4 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL.

别名

35 kDa inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; gp120 ELISA Kit; H4P ELISA Kit; IHRP ELISA Kit; Inter-alpha-inhibitor heavy chain 4 ELISA Kit; Inter-alpha-trypsin inhibitor family heavy chain-related protein ELISA Kit; Inter-alpha-trypsin inhibitor heavy chain H4 ELISA Kit; inter-alpha-trypsin inhibitor, heavy chain-like, 1 ELISA Kit; ITI heavy chain H4 ELISA Kit; ITI-HC4 ELISA Kit; ITIH4 ELISA Kit; ITIH4_HUMAN ELISA Kit; ITIHL1 ELISA Kit; OTTHUMP00000197120 ELISA Kit; OTTHUMP00000197121 ELISA Kit; OTTHUMP00000213834 ELISA Kit; OTTHUMP00000213869 ELISA Kit; PK-120 ELISA Kit; PK120 ELISA Kit; Plasma kallikrein sensitive glycoprotein 120 ELISA Kit; PRO1851 ELISA Kit

缩写

ITIH4

Uniprot No

Q14624

灵敏度

0.156 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Immunology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human ITIH4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		87
		Range %		82-97
1:2		Average %		97
		Range %		92-102
1:4		Average %		96
		Range %		90-104
1:8		Average %		97
		Range %		92-102

回收率

The recovery of human ITIH4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		106		98-110
EDTA plasma (n=4)		95		91-105

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   40 2.539 2.438 2.489 2.329   20 2.047 1.946 1.997 1.837   10 1.312 1.301 1.307 1.147   5 0.755 0.766 0.761 0.601   2.5 0.463 0.452 0.458 0.298   1.25 0.337 0.326 0.332 0.172   0.625 0.242 0.245 0.244 0.084   0 0.160 0.159 0.160

货期

3-5 working days