产品描述

This Human mTOR ELISA Kit was designed for the quantitative measurement of Human mTOR protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 pg/mL-100 pg/mL and the sensitivity is 0.39 pg/mL.

别名

dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) ELISA Kit; FK506 binding protein 12 rapamycin associated protein 1 ELISA Kit; FK506 binding protein 12 rapamycin associated protein 2 ELISA Kit; FK506 binding protein 12 rapamycin complex associated protein 1 ELISA Kit; FK506-binding protein 12-rapamycin complex-associated protein 1 ELISA Kit; FKBP rapamycin associated protein ELISA Kit; FKBP12 rapamycin complex associated protein ELISA Kit; FKBP12-rapamycin complex-associated protein 1 ELISA Kit; FKBP12-rapamycin complex-associated protein ELISA Kit; FLJ44809 ELISA Kit; FRAP ELISA Kit; FRAP1 ELISA Kit; FRAP2 ELISA Kit; Mammalian target of rapamycin ELISA Kit; Mechanistic target of rapamycin ELISA Kit; mTOR ELISA Kit; MTOR_HUMAN ELISA Kit; OTTHUMP00000001983 ELISA Kit; RAFT1 ELISA Kit; Rapamycin and FKBP12 target 1 ELISA Kit; Rapamycin associated protein FRAP2 ELISA Kit; Rapamycin target protein 1 ELISA Kit; Rapamycin target protein ELISA Kit; RAPT1 ELISA Kit; Serine/threonine-protein kinase mTOR ELISA Kit

缩写

mTOR

Uniprot No

P42345

灵敏度

0.39 pg/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Cell Biology

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human mTOR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:100		Average %		86
		Range %		80-92
1:200		Average %		98
		Range %		91-105
1:400		Average %		101
		Range %		92-110
1:800		Average %		93
		Range %		86-98

回收率

The recovery of human mTOR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		93		89-98
EDTA plasma (n=4)		97		90-100

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml OD1 OD2 Average Corrected 100 2.675 2.666 2.671 2.546 50 2.312 2.189 2.251 2.126 25 1.709 1.663 1.686 1.561 12.5 1.047 0.989 1.018 0.893 6.25 0.617 0.603 0.610 0.485 3.12 0.429 0.412 0.421 0.296 1.56 0.238 0.229 0.234 0.109 0 0.129 0.121 0.125

货期

3-5 working days