产品描述
IgM(免疫球蛋白M)是一种血液中存在的抗体类型,通常出现在初次感染和特定疾病后。
华美生物所提供的Fish immunoglobulin M (IgM) ELISA Kit属于ELISA检测试剂盒,采用竞争法定量检测鱼类血清、血浆、组织匀浆样本中的IgM,其灵敏度为1.25 μg/mL,检测范围为1.25 μg/mL-50 μg/mL。
缩写
IgM
灵敏度
1.25 μg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IgM in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
86-101 |
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| 1:2 |
Average % |
97 |
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| Range % |
90-104 |
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| 1:4 |
Average % |
95 |
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| Range % |
84-105 |
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| 1:8 |
Average % |
95 |
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| Range % |
91-99 |
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回收率
| The recovery of fish IgM spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-105 |
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| EDTA plasma (n=4) |
95 |
91-102 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
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| 50 |
0.122 |
0.124 |
0.123 |
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| 25 |
0.213 |
0.199 |
0.206 |
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| 11.5 |
0.401 |
0.427 |
0.414 |
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| 3.75 |
0.745 |
0.761 |
0.753 |
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| 1.25 |
1.375 |
1.402 |
1.389 |
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| 0 |
2.541 |
2.532 |
2.537 |
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货期
3-5 working days