产品描述
This Rat MMP2 ELISA Kit was designed for the quantitative measurement of Rat MMP2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.625 ng/mL-40 ng/mL and the sensitivity is 0.156 ng/mL .
别名
Mmp2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
缩写
MMP2
Uniprot No
P33436
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:5 | Average % | 92 | |
| Range % | 88-96 | |
| 1:10 | Average % | 93 | |
| Range % | 90-93 | |
| 1:20 | Average % | 89 | |
| Range % | 84-94 | |
| 1:40 | Average % | 96 | |
| Range % | 91-100 | |
回收率
| The recovery of rat MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 91 | 87-96 | |
| EDTA plasma (n=4) | 103 | 95-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 40 | 2.194 | 2.283 | 2.239 | 2.093 | | | 20 | 1.804 | 1.959 | 1.882 | 1.736 | | | 10 | 1.280 | 1.386 | 1.333 | 1.187 | | | 5 | 0.891 | 0.870 | 0.881 | 0.735 | | | 2.5 | 0.455 | 0.464 | 0.460 | 0.314 | | | 1.25 | 0.297 | 0.307 | 0.302 | 0.156 | | | 0.625 | 0.228 | 0.225 | 0.227 | 0.081 | | | 0 | 0.142 | 0.150 | 0.146 | | |
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货期
3-5 working days