产品描述
This Human MGAM ELISA Kit was designed for the quantitative measurement of Human MGAM protein in serum, plasma, tissue homogenates, urine. It is a Sandwich ELISA kit, its detection range is 3.12 μU/mL-200 μU/mL and the sensitivity is 0.78 μU/mL.
别名
4-alpha-glucosidase ELISA Kit; Alpha-glucosidase ELISA Kit; Glucan 1 ELISA Kit; Glucoamylase ELISA Kit; Maltase-glucoamylase; intestinal ELISA Kit; MGA ELISA Kit; MGA_HUMAN ELISA Kit; MGAM ELISA Kit; MGAML ELISA Kit
缩写
MGAM
Uniprot No
O43451
灵敏度
0.78 μU/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Others
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human α-Glucosidase in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:20 | Average % | 95 | | |
| Range % | 85-103 | | |
| 1:40 | Average % | 90 | | |
| Range % | 85-99 | | |
| 1:80 | Average % | 95 | | |
| Range % | 91-102 | | |
| 1:100 | Average % | 89 | | |
| Range % | 81-97 | | |
回收率
| The recovery of human α-Glucosidase spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 95 | 85-101 | | |
| EDTA plasma (n=4) | 93 | 87-99 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| µU/ml | OD1 | OD2 | Average | Corrected | | | | 200 | 2.127 | 2.112 | 2.120 | 1.913 | | | | 100 | 1.746 | 1.757 | 1.752 | 1.545 | | | | 50 | 1.442 | 1.417 | 1.430 | 1.223 | | | | 25 | 1.069 | 1.028 | 1.049 | 0.842 | | | | 12.5 | 0.835 | 0.843 | 0.839 | 0.632 | | | | 6.25 | 0.502 | 0.521 | 0.512 | 0.305 | | | | 3.12 | 0.347 | 0.354 | 0.351 | 0.144 | | | | 0 | 0.205 | 0.209 | 0.207 | | | |
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货期
3-5 working days