产品描述
This Mouse IL16 ELISA Kit was designed for the quantitative measurement of Mouse IL16 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .
别名
Il16 ELISA Kit; Pro-interleukin-16 [Cleaved into: Interleukin-16 ELISA Kit; IL-16 ELISA Kit; Lymphocyte chemoattractant factor ELISA Kit; LCF)] ELISA Kit
缩写
IL16
Uniprot No
O54824
灵敏度
1.95 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IL-16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 80-99 | |
| 1:2 | Average % | 96 | |
| Range % | 91-101 | |
| 1:4 | Average % | 95 | |
| Range % | 92-100 | |
| 1:8 | Average % | 98 | |
| Range % | 86-115 | |
回收率
| The recovery of mouse IL-16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 88 | 80-115 | |
| EDTA plasma (n=4) | 95 | 90-108 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 500 | 2.750 | 2.800 | 2.775 | 2.678 | | | 250 | 2.058 | 2.148 | 2.103 | 2.006 | | | 125 | 1.258 | 1.366 | 1.312 | 1.215 | | | 62.5 | 0.785 | 0.840 | 0.813 | 0.716 | | | 31.2 | 0.520 | 0.482 | 0.501 | 0.404 | | | 15.6 | 0.385 | 0.383 | 0.384 | 0.287 | | | 7.8 | 0.196 | 0.199 | 0.198 | 0.101 | | | 0 | 0.098 | 0.096 | 0.097 | | |
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货期
3-5 working days