产品描述
This Human IGFBP5 ELISA Kit was designed for the quantitative measurement of Human IGFBP5 protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
IBP 5 ELISA Kit; IBP-5 ELISA Kit; IBP5 ELISA Kit; IBP5_HUMAN ELISA Kit; IGF binding protein 5 ELISA Kit; IGF BP5 ELISA Kit; IGF-binding protein 5 ELISA Kit; IGFBP 5 ELISA Kit; IGFBP-5 ELISA Kit; IGFBP5 ELISA Kit; Insulin like growth factor binding protein 5 ELISA Kit; Insulin-like growth factor-binding protein 5 ELISA Kit
缩写
IGFBP5
Uniprot No
P24593
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | |
| Three samples of known concentration were tested twenty times on one plate to assess. |
| Inter-assay Precision (Precision between assays): CV%<10% | | |
| Three samples of known concentration were tested in twenty assays to assess. | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) |
| 1:100 | Average % | 92 |
| Range % | 84-95 |
| 1:200 | Average % | 105 |
| Range % | 97-108 |
| 1:400 | Average % | 94 |
| Range % | 88-97 |
| 1:800 | Average % | 98 |
| Range % | 94-101 |
回收率
| The recovery of human IGFBP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range |
| Serum (n=5) | 93 | 86-97 |
| EDTA plasma (n=4) | 93 | 88-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml | OD1 | OD2 | Average | Corrected | | 10 | 2.613 | 2.557 | 2.585 | 2.493 | | 5 | 1.804 | 1.818 | 1.811 | 1.719 | | 2.5 | 0.978 | 0.966 | 0.972 | 0.880 | | 1.25 | 0.561 | 0.584 | 0.573 | 0.481 | | 0.625 | 0.324 | 0.304 | 0.314 | 0.222 | | 0.312 | 0.251 | 0.234 | 0.243 | 0.151 | | 0.156 | 0.143 | 0.135 | 0.139 | 0.047 | | 0 | 0.091 | 0.093 | 0.092 | |
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货期
3-5 working days