产品描述
This Guinea pig IL10 ELISA Kit was designed for the quantitative measurement of Guinea pig IL10 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL .
别名
IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写
IL10
Uniprot No
Q9Z1Y5
灵敏度
7.81 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 90 | |
| Range % | 85-95 | |
| 1:2 | Average % | 96 | |
| Range % | 91-103 | |
| 1:4 | Average % | 95 | |
| Range % | 89-100 | |
| 1:8 | Average % | 97 | |
| Range % | 91-100 | |
回收率
| The recovery of guinea pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 96 | 89-101 | |
| EDTA plasma (n=4) | 97 | 91-105 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| pg/ml | OD1 | OD2 | Average | Corrected | | | 2000 | 2.894 | 2.903 | 2.899 | 2.780 | | | 1000 | 2.107 | 2.115 | 2.111 | 1.992 | | | 500 | 1.233 | 1.241 | 1.237 | 1.118 | | | 250 | 0.756 | 0.767 | 0.762 | 0.643 | | | 125 | 0.443 | 0.451 | 0.447 | 0.328 | | | 62.5 | 0.289 | 0.299 | 0.294 | 0.175 | | | 31.25 | 0.184 | 0.197 | 0.191 | 0.072 | | | 0 | 0.118 | 0.120 | 0.119 | | |
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货期
3-5 working days