产品描述

This Human KRT1 ELISA Kit was designed for the quantitative measurement of Human KRT1 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

别名

67 kDa cytokeratin ELISA Kit; CK-1 ELISA Kit; CK1 ELISA Kit; Cytokeratin-1 ELISA Kit; Cytokeratin1 ELISA Kit; EHK ELISA Kit; EHK1 ELISA Kit; Epidermolytic hyperkeratosis 1 ELISA Kit; EPPK ELISA Kit; Hair alpha protein ELISA Kit; K1 ELISA Kit; K2C1_HUMAN ELISA Kit; Keratin ELISA Kit; Keratin type II cytoskeletal 1 ELISA Kit; Keratin-1 ELISA Kit; Keratin1 ELISA Kit; KRT 1 ELISA Kit; Krt1 ELISA Kit; KRT1A ELISA Kit; NEPPK ELISA Kit; type II cytoskeletal 1 ELISA Kit; Type II keratin Kb1 ELISA Kit; Type-II keratin Kb1 ELISA Kit

缩写

KRT1

Uniprot No

P04264

灵敏度

0.039 ng/mL

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Signal Transduction

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human KRT1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
		Sample		Serum(n=4)
1:1		Average %		90
		Range %		87-93
1:2		Average %		85
		Range %		81-90
1:4		Average %		99
		Range %		95-104
1:8		Average %		97
		Range %		91-103

回收率

The recovery of human KRT1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type		Average % Recovery		Range
Serum (n=5)		87		82-91
EDTA plasma (n=4)		92		85-96

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average Corrected   10 2.862 2.898 2.880 2.727   5 1.991 1.999 1.995 1.842   2.5 1.172 1.198 1.185 1.032   1.25 0.640 0.674 0.657 0.504   0.625 0.425 0.436 0.431 0.278   0.312 0.353 0.368 0.361 0.208   0.156 0.260 0.268 0.264 0.111   0 0.152 0.154 0.153

货期

3-5 working days