产品描述
This Human KRT6A ELISA Kit was designed for the quantitative measurement of Human KRT6A protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.
别名
CK-6A ELISA Kit; CK-6D ELISA Kit; CK6A ELISA Kit; CK6C ELISA Kit; CK6D ELISA Kit; Cytokeratin-6A ELISA Kit; Cytokeratin-6D ELISA Kit; K2C6A_HUMAN ELISA Kit; K6A ELISA Kit; K6C ELISA Kit; K6D ELISA Kit; keratin 6A ELISA Kit; Keratin ELISA Kit; Keratin; type II cytoskeletal 6A ELISA Kit; Keratin-6A ELISA Kit; Krt6a ELISA Kit; KRT6C ELISA Kit; KRT6D ELISA Kit; type II cytoskeletal 6A ELISA Kit; Type-II keratin Kb6 ELISA Kit
缩写
KRT6A
Uniprot No
P02538
灵敏度
0.039 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Signal Transduction
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human KRT6A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |
| | Sample | Serum(n=4) | | |
| 1:1 | Average % | 87 | | |
| Range % | 82-91 | | |
| 1:2 | Average % | 84 | | |
| Range % | 80-88 | | |
| 1:4 | Average % | 103 | | |
| Range % | 98-108 | | |
| 1:8 | Average % | 90 | | |
| Range % | 85-94 | | |
回收率
| The recovery of human KRT6A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |
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| Sample Type | Average % Recovery | Range | | |
| Serum (n=5) | 98 | 94-103 | | |
| EDTA plasma (n=4) | 94 | 90-100 | | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |
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| ng/ml | OD1 | OD2 | Average | Corrected | | | | 10 | 2.098 | 2.102 | 2.100 | 1.955 | | | | 5 | 1.721 | 1.793 | 1.757 | 1.612 | | | | 2.5 | 1.382 | 1.421 | 1.402 | 1.257 | | | | 1.25 | 1.090 | 1.150 | 1.120 | 0.975 | | | | 0.625 | 0.695 | 0.714 | 0.705 | 0.560 | | | | 0.312 | 0.459 | 0.436 | 0.448 | 0.303 | | | | 0.156 | 0.267 | 0.262 | 0.265 | 0.120 | | | | 0 | 0.142 | 0.147 | 0.145 | | | |
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货期
3-5 working days