产品描述
LGALS3(Galectin-3)是一种β-半乳糖苷结合蛋白,在细胞增殖、凋亡、黏附和迁移等生理过程中发挥重要作用。LGALS3含量的变化与多种疾病,如肿瘤、心血管疾病和肝病等,的发生密切相关。
华美生物所提供的Human lectin-galactose binding-soluble 3,Lgals3 ELISA Kit属于ELISA检测试剂盒,采用双抗夹心法定量检测人血清、血浆、细胞培养上清、组织匀浆、唾液样本中的LGALS3,其灵敏度为0.156 ng/mL,检测范围为0.625 ng/mL-40 ng/mL。
别名
35 kDa lectin ELISA Kit; Carbohydrate binding protein 35 ELISA Kit; Carbohydrate-binding protein 35 ELISA Kit; CBP 35 ELISA Kit; CBP35 ELISA Kit; Gal-3 ELISA Kit; GAL3 ELISA Kit; Galactose-specific lectin 3 ELISA Kit; Galactoside-binding protein ELISA Kit; GALBP ELISA Kit; Galectin 3 internal gene,included ELISA Kit; Galectin-3 ELISA Kit; Galectin3 ELISA Kit; GALIG ELISA Kit; GBP ELISA Kit; IgE binding protein ELISA Kit; IgE-binding protein ELISA Kit; L 31 ELISA Kit; L 34 ELISA Kit; L-31 ELISA Kit; L-34 galactoside-binding lectin ELISA Kit; L31 ELISA Kit; Laminin-binding protein ELISA Kit; Lectin L-29 ELISA Kit; Lectin, galactose binding, soluble 3 ELISA Kit; LEG3_HUMAN ELISA Kit; LGALS2 ELISA Kit; LGALS3 ELISA Kit; MAC 2 antigen ELISA Kit; Mac-2 ELISA Kit; Mac-2 antigen ELISA Kit; MAC2 ELISA Kit; Macrophage galactose-specific lectin ELISA Kit; MGC105387 ELISA Kit
缩写
LGALS3
Uniprot No
P17931
灵敏度
0.156 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cell Biology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of human Lgals3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
88 |
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| Range % |
83-95 |
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| 1:2 |
Average % |
98 |
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| Range % |
91-104 |
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| 1:4 |
Average % |
92 |
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| Range % |
87-96 |
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| 1:8 |
Average % |
93 |
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| Range % |
88-100 |
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回收率
| The recovery of human Lgals3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
93 |
86-97 |
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| EDTA plasma (n=4) |
102 |
94-107 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 40 |
2.346 |
2.331 |
2.339 |
2.185 |
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| 20 |
1.659 |
1.676 |
1.668 |
1.514 |
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| 10 |
1.139 |
1.151 |
1.145 |
0.991 |
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| 5 |
0.699 |
0.678 |
0.689 |
0.535 |
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| 2.5 |
0.498 |
0.487 |
0.493 |
0.339 |
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| 1.25 |
0.336 |
0.343 |
0.340 |
0.186 |
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| 0.625 |
0.227 |
0.212 |
0.220 |
0.066 |
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| 0 |
0.156 |
0.152 |
0.154 |
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货期
3-5 working days