产品描述

This Human LDHA ELISA Kit was designed for the quantitative measurement of Human LDHA protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 31.25 mU/mL-2000 mU/mL and the sensitivity is 7.81 mU/Ml.

别名

Cell proliferation-inducing gene 19 protein ELISA Kit; GSD11 ELISA Kit; L lactate dehydrogenase B chain ELISA Kit; L-lactate dehydrogenase A chain ELISA Kit; Lactate dehydrogenase A ELISA Kit; Lactate dehydrogenase B ELISA Kit; Lactate dehydrogenase c variant 1 ELISA Kit; Lactate dehydrogenase c variant 3 ELISA Kit; Lactate dehydrogenase c variant 4 ELISA Kit; Lactate dehydrogenase C4 ELISA Kit; Lactate dehydrogenase H chain ELISA Kit; Lactate dehydrogenase M ELISA Kit; LDH A ELISA Kit; LDH B ELISA Kit; LDH H ELISA Kit; LDH heart subunit ELISA Kit; LDH M ELISA Kit; LDH muscle subunit ELISA Kit; LDH-A ELISA Kit; LDH-M ELISA Kit; LDH1 ELISA Kit; ldha ELISA Kit; LDHA_HUMAN ELISA Kit; LDHBD ELISA Kit; LDHM ELISA Kit; MS1111 ELISA Kit; PIG19 ELISA Kit; Proliferation inducing gene 19 ELISA Kit; Proliferation-inducing gene 19 ELISA Kit; Renal carcinoma antigen NY REN 46 ELISA Kit; Renal carcinoma antigen NY-REN-59 ELISA Kit; TRG 5 ELISA Kit; TRG5 ELISA Kit

缩写

LDHA

Uniprot No

P00338

灵敏度

7.81 mU/Ml

反应时间

1-5h

样本体积

50-100ul

检测波长

450 nm

研究领域

Metabolism

测定原理

quantitative

精密度

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

线性度

To assess the linearity of the assay, samples were spiked with high concentrations of human LDHA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

88

Range %

81-92

1:2

Average %

99

Range %

94-102

1:4

Average %

101

Range %

97-105

1:8

Average %

94

Range %

88-98

回收率

The recovery of human LDHA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type			Average % Recovery				Range
Serum (n=5)			93				89-96
EDTA plasma (n=4)			96				94-98

标准曲线

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml OD1 OD2 Average Corrected 2000 2.101 1.952 2.027 1.914 1000 1.676 1.734 1.705 1.592 500 1.252 1.238 1.245 1.132 250 0.768 0.758 0.763 0.650 125 0.566 0.568 0.567 0.454 62.5 0.328 0.321 0.325 0.212 31.25 0.179 0.185 0.182 0.069 0 0.114 0.112 0.113

货期

3-5 working days