产品描述
This Bovine IL1A ELISA Kit was designed for the quantitative measurement of Bovine IL1A protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 250 pg/mL-4000 pg/mL and the sensitivity is 250 pg/mL.
别名
IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
缩写
IL-1
Uniprot No
P08831
灵敏度
250 pg/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Immunology
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<15% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<15% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
85-94 |
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| 1:2 |
Average % |
92 |
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| Range % |
87-97 |
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| 1:4 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:8 |
Average % |
98 |
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| Range % |
90-102 |
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回收率
| The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-98 |
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| EDTA plasma (n=4) |
89 |
83-96 |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
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| 4000 |
0.147 |
0.141 |
0.144 |
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| 2000 |
0.296 |
0.279 |
0.288 |
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| 1000 |
0.431 |
0.467 |
0.449 |
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| 500 |
0.782 |
0.823 |
0.803 |
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| 250 |
1.862 |
1.826 |
1.844 |
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货期
3-5 working days