产品描述
This Dog MMP3 ELISA Kit was designed for the quantitative measurement of Dog MMP3 protein in serum, plasma, cell culture supernates, urine, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 1.56 ng/mL-100 ng/mL and the sensitivity is 0.39 ng/mL .
别名
MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit
缩写
MMP3
Uniprot No
Q6Y4Q5
灵敏度
0.39 ng/mL
反应时间
1-5h
样本体积
50-100ul
检测波长
450 nm
研究领域
Cancer
测定原理
quantitative
精密度
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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线性度
| To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 92 | |
| Range % | 88-96 | |
| 1:2 | Average % | 89 | |
| Range % | 85-96 | |
| 1:4 | Average % | 93 | |
| Range % | 89-97 | |
| 1:8 | Average % | 99 | |
| Range % | 92-105 | |
回收率
| The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 95 | 91-99 | |
| EDTA plasma (n=4) | 92 | 87-96 | |
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标准曲线
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| ng/ml | OD1 | OD2 | Average | Corrected | | | 100 | 2.410 | 2.545 | 2.478 | 2.284 | | | 50 | 1.960 | 1.982 | 1.971 | 1.777 | | | 25 | 1.595 | 1.563 | 1.579 | 1.385 | | | 12.5 | 1.177 | 1.201 | 1.189 | 0.995 | | | 6.25 | 0.786 | 0.789 | 0.788 | 0.594 | | | 3.12 | 0.458 | 0.462 | 0.460 | 0.266 | | | 1.56 | 0.278 | 0.262 | 0.270 | 0.076 | | | 0 | 0.193 | 0.195 | 0.194 | | |
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货期
3-5 working days